Only lines with voice work for me, even though there are plenty of great unvoiced lines across games. Sorted by game... I think this post certainly puts me of a generation.

"My emperor! I've failed youuuu!"
"Fox, get this guy off me!"
"You're good, but I'm BETTER!"
"Never give up, trust your instincts!"
"IF THIS DOES NOT WORK!"

Honestly one of the most quoteable games ever made.

"Stay a while and listen."

"My hotel's as clean as an Elven arse!"
"Whaaaat?!"
"Tiax shall smite ye."
"Heya, it's me Imoen."

"MASSACRE!"
"Not yet!"
"hyut hyut hyut"

"My fists never betray me!"
"Don't stand behind me."
"I'm gonna sleep well tonight!"
"The feeling... It's... coming back!"
"Good vibrations!"

"Hello brother! [...] I live on in this arm!"
"Laugh, and grow fat!"
"What was that just now?"

Love a good ham. We need more of them in video games.

"I'mma Luigi, number 1."
"Falcon PUNCH!"

"You're going the wrong way!"
"Retired!"

"You require more vespene gas!"
"All crews operational." / "Battlecruiser operational." ?
"En'taro Adun executor."
"I do this for Auir, not you!"
"Your base in under attack."

"So what shall it be? Will you join the Unity or will you die here? Join... DIE! Join... DIE!"

"Doctor required in inflator room."

"[...] to the last place uncorrupted by Capitalism! SPACE!"